To visualize thymocytes expressing Runx3d mRNA, we generated a reporter allele (Runx3dYFP) by replacing the first coding exon used by the Runx3 distal promoter with sequence encoding yellow fluorescent protein (YFP; Supplementary Fig. 1a online). In accordance with published analyses of Runx protein … See more To visualize ThPOK expression in individual differentiating thymocytes, we generated a green fluorescent protein (GFP)-expressing 'knock-in' allele of ThPOK by replacing all of … See more To determine whether there was cross-regulation of ThPOK and Runx3 during thymocyte lineage specification, we examined expression of the ThPOK and Runx3d reporters in the absence of one or the other factor. … See more Our data indicated a correlation between Runx3 derepression and redirection of MHC class II–restricted thymocytes to the cytotoxic lineage. To determine whether Runx complexes were required for lineage redirection occurring … See more Our data indicated that ThPOK expression after MHC class II–restricted positive selection prevented expression of Runx3d. However, our analysis of ThPOKGFP expression showed … See more WebFigure 2 Runx3d and ThPOK reporter expression in developing thymocytes. (a) Distal promoter–derived Runx3 expression assessed with the Runx3dYFP reporter in thymocyte subpopulations (as defined in Supplementary Fig. 2). (b) ThPOKGFP reporter expression in developing thymocytes. Numbers adjacent to outlined areas (a,b) indicate percent YFP+ …
Critical Role for TCR Signal Strength and MHC Specificity in …
WebThe R3-5G4 monoclonal antibody specifically binds to Runt-related transcription factor 3 (RUNX3) which is also known as Acute myeloid leukemia 2 protein (AML2), Core-binding factor subunit alpha-3 (CBFA3), and Polyomavirus enhancer-binding protein 2 alpha C subunit (PEBP2aC). RUNX3 is a member of the RUNX transcription factor family which … WebMay 31, 2024 · Runx3d further functions as a transcriptional silencer of Zbtb7b (which encodes Thpok) expression (158, 159). The reestablishment of TCR signals and the increased expression of Eomes and genes associated with granule- and interferon-γ (IFN-γ)–mediated cytotoxic functions induce differentiation into mature CD8 single-positive … fern wright columbia mo
From inception to output, Tcf1 and Lef1 safeguard development …
WebThe data shown are pooled from two independent experiments. (G) The frequency of ThPOK hi cells (mean +- SEM) after 3 d in in vitro culture of CD4 + T cells in the presence of TGF … WebDec 1, 2012 · TLDR. A new autoregulatory loop whereby ThPOK binds to the SilThPOK to maintain its own long-term expression in CD4 T cells is described, crucial for tissue … WebThe data shown are pooled from two independent experiments. (G) The frequency of ThPOK hi cells (mean +- SEM) after 3 d in in vitro culture of CD4 + T cells in the presence of TGF-beta, RA, ... ( b ) Real time PCR analysis of ThPOK, Runx3 and Runx3d mRNA expressions in CD4 + CD8 int thymocytes. Results are presented as relative expression of ... deliver food to my location