Fluxor herg assay h9c2
WebThe Predictor™ hERG kit from Invitrogen is a homogeneous fluorescent assay that uses a simple add-and-read format. The assay is based on the principle of fluorescence … WebMar 5, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2. Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for …
Fluxor herg assay h9c2
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WebMar 17, 2024 · This assay measures hERG channel activity using thallium ions as surrogate ions. Prior to the experiment, hERG-U2OS or hERG-HEK293 stable cell lines expressing … WebFluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturers protocol. • Fluorescence signals were elicited by 1mM Tl + and 10 mM K + and detected on FLIPR …
WebThe FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel … WebThe FluxOR assay was performed using the washing method recommended by the supplier, and bottom fluorescence intensity was assessed by end-point readout in the SynergyH1 microplate reader …
WebThe hERG assay, an in vitro electrophysiologic assay, has become routine since its development in the 1990s. It is one of the recommended studies in the ICH S7B regulatory guideline. This is a voltage clamp assay where a cultured mammalian cell (usually human embryonic kidney, or HEK cell) is transfected with hERG DNA. WebFluxOR™ thallium assay has been widely recognized for hERG activity test. This assay is optically based and can be performed on 96-well or 384-well plates as a homogenous assay for high-throughput screening (HTS) …
WebApr 8, 2024 · ATP produced by H9c2 cells was assessed by the bioluminescence assay kit (Beyotime Biotechnology, Shanghai, China). In brief, the concentration of ATP was assessed by adding 20 μL of the supernatant into 100 μL of luciferase reagent; the luciferase present in the reagent catalyzes the production of luminescence from ATP and …
WebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … duty drawback under which headWebwater baths appear to act as hERG blockers and can reduce assay performance or cause complete assay failure. 4.1 Predictor™ hERG Tracer Red Store Predictor™ hERG … in addition academic writingWebFluxxor - Home. Fluxxor is a set of tools to facilitate building JavaScript data layers using the Flux architecture by reifying many of the core Flux concepts. It works particularly well … in addition alternative wordsWebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 μL/well) and ... duty drawback specialistWebMTT assay was carried out using H9c2 cells to determine a proper dose range for the designed assay. The ECG measurement revealed that intravenous injection of 4-AcO-DET at 2.0 mg/kg and 4-HO-MET at 1.5 mg/kg prolonged the QTc interval in SD rats. In addition, hERG potassium channels in Chinese hamster ovary cells treated test substances were ... in addition as well crossword clueWebs of hERG inhibitors determined in separate tests. N=4. FLIPR Potassium Kit-4 -2 0 2 0 1 3 Dofetilide Terfenadine Pimozide Log[inhibitor] mM) FluxOr Kit-4 -2 0 2 0.0 0.2 0.4 0.6 0.8 1.0 Dofetilide Terfenadine Pimozide Log[inhibitor] mM Compound) IC 50 (nM) FLIPR Potassium Assay 50) FluxOR Assay Dofetalide 39 53 Pimozide 135 195 Terfenadine … in addition becauseWebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye … duty engineer hiring in singapore