Flp-in 293 cells

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August undefined, 2024

WebArticle Snippet: The Flp-In™ T-REx™ 293 Cell Line (HEK293) was procured from Thermo Fisher Scientific and maintained in DMEM (4.5 g/L glucose) with L-Gln and sodium pyruvate (Nacalai Tesque, 08458-45), supplemented with 10% FBS, uridine (50 mg/L), blasticidin … WebIn hES cells where LIN28 is expressed at high levels, we performed CLIP-seq with an antibody that specifically recognizes the endogenous protein (Figures 1A and S1A).To model the reactivation of LIN28 expression observed in many cancer cells, we generated a stable Flp-In HEK293 cell line that constitutively expresses a C-terminal V5-tagged …

T-REx System - Thermo Fisher Scientific

WebInvitrogen™ Flp-In™ T-REx™ 293 Cell Line. Cells contain single stably integrated FRT site at transcriptionally active genomic locus. Includes: 1 x 10 7 cells are supplied frozen in 1mL of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. WebApr 1, 2016 · Symbols and abbreviations are as described in Fig. 1. (E) Flp-In-293 cells were transiently transfected with the indicated GPR37 constructs for 24 h, and the anti-Myc-antibody-labelled full-length receptors were analysed by confocal microscopy as … cullum\\u0027s attaboy san antonio

Genome targeting by hybrid Flp-TAL recombinases

WebJan 1, 2012 · We described here a simple method to display full-length antibody on Flp-In 293 cells (F293) by transient transfection of a single vector into the cells. The full-length antibodies can easily be detected on cell surface by flow cytometry 36–48 h post-transfection. 2 Materials. WebCell Line Catalog no. Flp-In™-293 R750-07 Flp-In™-CV-1 R752-07 Flp-In™-CHO™ R758-07 Flp-In™-BHK R760-07 Flp-In™-3T3 R761-07 Flp-In™-Jurkat R762-07 All cell lines are supplied as one vial containing 1 × 107 frozen cells in 1 mL of Freezing Medium. … WebMar 22, 2024 · The 17 used localization markers in both cell lines align well with their respective localization, when processed against the Flp-In T-REx 293 reference molecular context proteome map ... east hanover boe

Using the Flp-In™ T-Rex™ system to regulate GPCR expression

Flp-In™-293 Cell Line - Thermo Fisher Scientific

WebJul 4, 2024 · In the present study, we employed Flp-In-293 cells with the Flp-In™ system to establish cells expressing WT or non-synonymous SNP variants of human ABCC4 (Figure 1 and Table 1). Flp-In-293 cells were transfected with the ABCC4 cDNA, which integrated … WebRNA-seq of the NE cells homozygous for W1282X showed that CFTR transcript level was reduced to 1.7% of WT (p-value: 4.6e-3). Negligible truncated CFTR protein was generated by Flp-In 293 cells stably expressing the W1282X EMG even though CFTR transcript was well above levels observed in the parents and proband. east hanover board of healthWebT-REx™ Cell Lines For your convenience, Invitrogen has available three mammalian cells lines that stably express the Tet repressor. T-REx™-293 cells and T-REx™-HeLa cells express the Tet repressor from pcDNA™6/TR and should be maintained in medium containing blasticidin. T-REx™-U2OS cells express east hanover bowling lanes

"WebFlp-In ™ T-REx -293 cell line. Complete Medium [Antibiotic] Freezing Medium D-MEM (high glucose) 10% FBS 2 mM L-glutamine 1% Pen-Strep (optional) 100 μg/mL Zeocin™ 15 μg/mL blasticidin 90% complete medium 10% DMSO Important Guidelines Consider the … " - Flp-in 293 cells

Flp-in 293 cells

Flp-In System: For Generating Constitutive Expression Cell …

WebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In …

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WebJun 6, 2015 · I am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... WebApr 6, 2024 · As most GBA1 mutations lead to a misfolded enzyme that is rapidly degraded via ER-associated degradation by the proteasome 11,12, we generated stable Flp-In™T-REx™-HEK 293 cell lines ...

WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture … WebFeb 4, 2024 · inducible cell lines to express either hnRNPA1 wild type or D262V mutant epitope-tagged cDNAs. These cells were generated using FLP-recombinase mediated integration in 293 cells, while expressing endogenous wild type hnRNPA1. This experimental set up was initially designed to mimic ALS patients carrying a heterozygous …

WebOct 15, 2024 · To test the integration activity of the Flp-TAL recombinases, we transfected HEK-293 cells with the vectors that express FV61-TAL, FV63-TAL, or FV71-TAL and the corresponding pTarget variant. 48 h ... WebCell Line Source Amount Catalog no. Flp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster ovary 1 × 107 cells, frozen R758–07 Flp-In™-BHK …

WebThe Flp-In™ T‑REx™-293 cell line is derived from 293 human embryonic kidney cells (Graham et al., 1977). The 293 parental cell line was obtained from the American Type Culture Collection (ATCC®). For more information about the 293 parental cell line, see …

WebNov 8, 2016 · The FH-AKAP95 and FH-AKAP95 (101–692) cell lines were made by co-transfecting Flp-In-293 cells (f293 cells, Invitrogen) with pOG44 plasmid and either an FH-AKAP95-pcDNA5 or an FH-AKAP95 (101 ... east hanover building departmentWebE , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA immunoprecipitates were blotted for CIAO3, CIA targeting complex components, and the CIA substrate CDKAL1. cullunghutti early learning centreWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. east hanover campus mapWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. These cells contain a single stably integrated FRT site at a trans cullwaterWebJan 6, 2024 · I am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... east hanover bowlingWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... cull wandWebApr 1, 2024 · 5 Flp-In 293 T-Rex cells (Therm o Fisher Scientific, #R78007) were grown in media composed of Dulbecco’s modified E agle’s medium (DMEM, Sigma– Aldrich), 10% fetal bovine serum cull wand essence