Cochlear whole mount
WebThe meaning of COCHLEA is a hollow tube in the inner ear of higher vertebrates that is usually coiled like a snail shell and contains the sensory organ of hearing. WebMar 5, 2013 · Mouse Cochlear Whole Mount Immunofluorescence. This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole …
Cochlear whole mount
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WebFeb 1, 2002 · Micro Dissecting Spring Scissors used to hemisect the cochlea into its superior and inferior parts and to trim slightly the lateral wall. (We found those with 3 mm …
WebCochlear Whole-Mount Preparation Isolated mice and rat cochleae were perfused with 4% paraformaldehyde and kept overnight at 4°C in the same solution for fixation. Cochleae were then decalcified in 100 mM EDTA … WebAug 21, 2013 · Cochlear histopathology After final physiological testing, cochleas were processed as immunostained whole mounts or osmium-stained, plastic-embedded sections to quantify hair cells, spiral ganglion cells, and synaptic structures, i.e., presynaptic ribbons, postsynaptic glutamate receptors, and cochlear-nerve terminal boutons under IHCs.
WebDec 9, 2024 · Immunohistochemistry on cochlear whole-mount preparations. Cochlear whole-mounts were rehydrated in a descending methanol/PBS series. For the first permeabilization step, whole-mounts were treated ... WebJul 6, 2024 · The cochlea is one of two main structures that make up the inner ear. The inner ear is located behind the eardrum and deep within the middle ear. The other structures …
Webcochlea [kok´le-ah] a spiral tube shaped like a snail shell, forming part of the inner ear; it is the essential organ of hearing. adj., adj coch´lear. The cochlea is filled with fluid and is …
WebMay 5, 2009 · Immunocytochemistry, Western-blot analysis and cochlear whole mount staining were carried out as described (Senften et al., 2006). Immunogold electron microscopy. All experiments involving animals and the maintenance of animals were carried out in accordance with institutional guidelines. Animals were anesthetized and perfused … crypt organoidWebDec 13, 2024 · Neuronal lesions were quantified in cochlear whole-mount preparations combined with microsections across the habenular perforate (HP) openings to achieve a clear picture of the topographic relationship between neuronal damage and HC loss. Multiple immunostaining methods were employed to differentiate the two types of afferent … crypt pe twitterWebJun 12, 2013 · To obtain adult cochlear whole-mount preparations, temporal bones were dissected and the cochleas were slowly perfused through the oval window with 4% paraformaldehyde (PFA) in PBS, postfixed for 2 h at room temperature, and decalcified in 150 m m EDTA for 5–7 d at 4°C before further dissection. crypt password generatorWebJun 29, 2016 · Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients. Brain Res Brain Res Protoc 9: 65-76 . Google Scholar Lewin GR, Barde YA. 1996. Physiology of the neurotrophins. Annu Rev Neurosci 19: 289-317 . Google Scholar Liberman MC. 1980. crypt password matchWebAug 12, 2024 · Summary. Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection … crypt password match エラーWebOct 7, 2024 · GFP expression in cochlear whole mount was analyzed and auditory brainstem response (ABR) tests were conducted one month after virus injection (for each animal, only left cochlea was injected and... crypt parva lightingWebJan 1, 2016 · Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea. The organ of Corti, housed in the cochlea of the inner ear, contains … crypt password